Abstract Details

Title MicroRNA Deregulation Is Involved in Microglial Activation in the Pilocarpine Induced Epilepsy Model

Topic Epilepsy

Presentation(s) IN9 - (-)

Poster/Presentation
Number 003

Objective

Background MicroRNAs regulate gene expression and are important in many key biological functions such as development, oncogenesis and inflammation. One microRNA, miR-124, is known to be expressed exclusively in the central nervous system and has been implicated in the maintenance of microglial cells in a non-activated, or quiescent, state. It is well known that activation of microglia must be properly and tightly regulated to maintain normal tissue homeostasis, avoiding tissue damage.

Design/Methods We obtained total RNA from hippocampus of animals at 1, 3, 6, and 24 hours, as well as 5 days after induction of status epilepticus (SE), and from control animals. We quantified expression of miR-124 by quantitative real-time PCR. Only tissue obtained from rats which presented SE were used in our experiments.

Results We found that expression of miR-124 significantly decreased after SE, about 60%, in comparison with control samples. MiR-124 expression was decreased already at 1h after SE and remained low until 5 days after SE.

Conclusions Our results indicate that regulation by microRNA, especially by miR-124, can play a role in microglia activation after SE induced by pilocarpine. Thus, opening the possibility of using microRNA-based therapies in epilepsy.

Authors/Disclosures
PRESENTER	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
Iscia Lopes-Cendes, MD, PhD (University of Campinas - UNICAMP)	No disclosure on file
Charles Yonan, PharmD (Avanir Pharmaceuticals)	No disclosure on file

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