Abstract Details

Title Development and Characterization of Light-Triggered Sleep-Deprivation Preclinical Migraine Model in Females and Males

Topic Headache

Presentation(s) N5 - Neuroscience in the Clinic: Melatonin and Disrupted Sleep in Neurologic Disorders (3:15 PM-3:30 PM)

Poster/Presentation
Number 001

Objective The purpose of this study was to develop a preclinical model for measuring sleep deprivation-induced migraine.

Background Sleep deprivation is the second most common migraine trigger. The mechanisms for sleep deprivation-induced migraines are not fully understood, partly because no standardized preclinical model exists for measuring sleep deprivation-induced migraines.

Design/Methods First, 13 wild-type male mice were habituated to baseline of no reaction to 0.6g von Frey filament stimulation on periorbital area. To trigger sleep deprivation, we adapted a procedure used to induce rodent sleep dysregulation. From 6am to 6pm, 150-200 lux of continuous light was applied to mice. From 6pm to 6am, 5 lux of continuous dim light was applied. Ambulation and rest activity were measured using SOF-812 Activity Monitor machines. Mice experienced dim light at night for one, three, or five continuous days and were randomly assigned to the machines during the night or day for a maximum of 12 hours per 24-hour period. After the experiment concluded, periorbital allodynia was measured using von Frey filaments at 1, 3, 5, 8, and every 3 days subsequently until mice returned to baseline.

Results There was a direct relationship between duration of dim light-triggered sleep deprivation and days of significant periorbital allodynia relative to baseline (P<0.05). All experimental groups exhibited at least one day of periorbital allodynia. In addition, activity level differences between night and day decreased, indicating sleep dysregulation.

Conclusions This preclinical model could provide a way to measure sleep deprivation-induced migraine. Next, experiments will be conducted using triptans and CGRP antagonists to confirm migraine-like pain in female and male mice. After validation, this model could be used in future studies on sex-dependent cell plasticity and underlying mechanisms at periphery (dura) and centrally (brain stem, hypothalamus, and thalamus). A set of these studies could reveal targets for new pharmacologic treatments of sleep deprivation-induced migraine.

Authors/Disclosures
Skyler Kanegi (Cincinnati Children's Hospital) PRESENTER	Mr. Kanegi has nothing to disclose.

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