Abstract Details

Title Autoantibody Reactivities in Serum and Cerebrospinal Fluid of Multiple Sclerosis Patients Detected with Peptide Microarrays

Topic MS and Related Diseases

Presentation(s) P03 - (-)

Poster/Presentation
Number 229

Objective

Background BACKGROUND: Several studies already identified autoantibody protein targets in serum and cerebrospinal fluid (CSF) of MS patients. Most antibody reactivities were only described to be specific for a subgroup of patients and still less is known about the true antigen epitopes.

Design/Methods DESIGN/METHODS: With informed consent, we studied antibody-peptide reactivities in serum and CSF of 10 patients with relapsing-remitting MS (RRMS), 10 patients with primary-progressive MS (PPMS) and 10 controls with other neurological diseases (OND). Peptides spotted on the microarrays were derived from diverse antigen candidates, including 40 proteins described in the literature as autoantigens in MS. In total, each microarray contained 6158 different peptides 10-20 amino acids in length.

Results RESULTS: The antibody signatures of the matched serum and CSF samples correlated clearly, though fewer peptides were found to be bound by IgG antibodies in CSF than in serum (in average >40 and >190 peptides per sample, respectively). Compared to the controls, antibody responses against >40 peptides were stronger in MS patients, even more so in the PPMS subgroup. Among the best discriminating epitopes was a part of a S100 calcium binding protein, which showed significant reactivities in 60% of the MS serum samples and none of the controls. Another interesting epitope was from a small heat shock protein, for which we observed high signal intensities for the CSF but not for the serum samples of 4 PPMS patients.

Conclusions CONCLUSIONS: In this large-scale screening, we found that the antibody repertoire against the selected linear peptides differs a lot between the patients. Several antigen reactivities were significantly elevated in individual MS patients, albeit not specific for MS in general. Some peptides were primarily recognized by intrathecally produced antibodies, which makes them attractive for deeper investigations.

Authors/Disclosures
Michael Hecker, PhD (Universitatsmedizin Rostock, Klinik Fur Neurologie) PRESENTER	No disclosure on file
Brigitte Paap (University of Rostock)	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
Rohit Bakshi, MD, FAAN	Dr. Bakshi has received personal compensation in the range of $10,000-$49,999 for serving as a Consultant for EMD Serono. Dr. Bakshi has received personal compensation in the range of $5,000-$9,999 for serving as a Consultant for Sanofi. The institution of Dr. Bakshi has received personal compensation in the range of $10,000-$49,999 for serving as an Editor, Associate Editor, or Editorial Advisory Board Member for Journal of Neuroimaging. The institution of Dr. Bakshi has received research support from Bristol Myers Squibb. The institution of Dr. Bakshi has received research support from EMD Serono. The institution of Dr. Bakshi has received research support from Novartis.

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