Abstract Details

Title Patients with Multiple Sclerosis Present an Unaltered Regulatory B Cell Frequency, Phenotype and Function

Topic MS and Related Diseases

Presentation(s) P03 - (-)

Poster/Presentation
Number 226

Objective

Background BACKGROUND: Multiple Sclerosis (MS) is a disease of the Central Nervous System (CNS) classically characterized by the recruitment of T cells into the CNS. However, some data support a role for B cells in its physiopathology. Recently it has emerged that subsets of B cells secreting IL-10 regulate disease symptoms in mouse models of inflammation.

Design/Methods DESIGN/METHODS: Sixty-three MS patients, untreated for at least 3 months and 58 age-matched HV were included in this study. Phenotype and IL-10 secretion by B cells were studied after stimulation with CD40L and ODN. Their suppressive properties on CD4+CD25-T cell proliferation were measured after 3 days of culture.

Results RESULTS: We report on an absence of differences in the frequencies of the various B cell subsets, as well as the ability of B cells to secrete IL-10 after 5h and 48h of stimulation in MS compared to HV. Analysis of the IL-10+ B cells phenotype revealed mainly a memory CD24+CD27+ phenotype, even though both naive and immature subsets were also able to secrete IL-10. Prestimulated B cells from MS and HV inhibited CD4+CD25- T cell proliferation with a similar efficiency by a contact-dependent mechanism, independently of IL-10 and TGF-?.

Conclusions CONCLUSIONS: Altogether, our data show that regulatory B cells have conserved frequency and function in the blood of patients with MS, suggesting that they do not contribute to the physiopathology of the disease.

Authors/Disclosures
Laure Michel PRESENTER	No disclosure on file
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	No disclosure on file
Jeffrey A. Cohen, MD (Cleveland Clinic)	Dr. Cohen has received personal compensation in the range of $10,000-$49,999 for serving as a Consultant for Convelo. Dr. Cohen has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Astoria. Dr. Cohen has received personal compensation in the range of $5,000-$9,999 for serving as a Consultant for Bristol Myers Squibb. Dr. Cohen has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Viatris. Dr. Cohen has received personal compensation in the range of $500-$4,999 for serving as a Consultant for PSI. Dr. Cohen has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Shionogi. Dr. Cohen has received personal compensation in the range of $500-$4,999 for serving on a Scientific Advisory or Data Safety Monitoring board for Celltrion.
	No disclosure on file
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Sandrine Wiertlewski (Hop Guillaume Et Rene Laennec)	No disclosure on file
	No disclosure on file
	No disclosure on file
David Laplaud	No disclosure on file
	No disclosure on file

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