Abstract Details

Title The Effects of UV Sterilization of a Poly(Caprolactone-Urethane) Biomaterial on Rodent Brain and Spinal Cord Neural Progenitor Cell Biocompatibility

Topic Neural Repair/Rehabilitation

Presentation(s) P03 - (-)

Poster/Presentation
Number 262

Objective

Background BACKGROUND: Repair of the injured spinal cord will require a combination of therapeutic strategies, and growth factor filled biodegradable guidance channels that can encapsulate an area of spinal cord injury are one means of facilitating combination therapy. Translation to clinical use requires testing of the effect of sterilization methods on these biomaterials.

Design/Methods DESIGN/METHODS: Adult female Sprague-Dawley rat subventricular zone brain tissue and the ependymal region spinal cord tissue were extracted. Neurospheres were seeded into 96-well plates and fed with plain media (controls) or media that was incubated with discs of UV sterilized poly(caprolactone-urethane) biomaterial. Spheres were differentiated at 7, 14 and 21 day time points and then fixed in PFA for confocal microscopy imaging. Bright field images were taken to measure sphere diameter.

Results RESULTS: Control spheres demonstrated a traditional linear growth pattern with average brain sphere sizes of 148.50±28.33[mu]m, 218.55±56.44[mu]m, and 234.03±76.18[mu]m, and average spine sphere sizes of 162.93±51.25[mu]m, 257.32±87.97[mu]m, and 331.44±83.59[mu]m at 7, 14 and 21 days respectively. In the biomaterial condition however, the sphere growth was slowed with average brain sphere sizes of 148.34±25.24[mu]m, 162.12±36.96[mu]m (26% smaller, p<0.001), and 156.51±43.26[mu]m (33% smaller, p<0.001), and spine sphere sizes of 139.58±37.60[mu]m, 138.67±45.35[mu]m (46% smaller, p<0.001), and 175.81±43.28[mu]m (47% smaller, p<0.001) at 7, 14 and 21 days respectively.

Conclusions CONCLUSIONS: Unlike previous experiments with gamma sterilized poly(caprolactone-urethane) biomaterial which demonstrated biocompatibility, UV sterilization resulted in limited neurosphere growth. The UV treatment may have damaged the chemical bonds of the biomaterial to produce a more rapid breakdown and creation of toxic by-products. Future testing of this biomaterial will hopefully assess sterilization methods that are biocompatible and facilitate spinal cord injury repair.

Authors/Disclosures
Stahs Pripotnev PRESENTER	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
Laura J. Julian, PhD (Genentech)	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file

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