Abstract Details

Title Tolerogenic Dendritic Cells in Experimental Autoimmune Encephalomyelitis as a Tool before Its Translation to MS Patients

Topic MS and Related Diseases

Presentation(s) P05 - (-)

Poster/Presentation
Number 171

Objective

Background BACKGROUND: Specific cell therapy with tolerogenic Dendritic Cells (tolDCs) loaded with autoantigens is a promising tool for the attenuation of pathogenic T cells in autoimmune diseases such as Multiple Sclerosis (MS). Before its translation to patients, it is important to better characterize its efects on the EAE model.

Design/Methods DESIGN/METHODS: Chronic EAE was induced in C57BL/6 mice by s.c. immunization with MOG40-55 emulsified in complete Freund's adjuvant. Pertussis Toxin was injected i.v. at days 0 and 2 post-immunization. Clinical score was daily measured based on tail/leg paralysis. TolBM-DCs were in vitro differenciated with GM-CSF in the presence of vitamin D3 (VD3) for 8 days. On day 7, maturation was induced with LPS. Viability, efficiency of differentiation and phenotype of tolBM-DCs were evaluated. To assess antigen specificity, tolBM-DCs were pulsed with MOG40-55, after the maturation stimulus (day 7) for 18h and cocultured with syngeneic splenocytes from mice with established EAE.

Results RESULTS: TolBM-DCs displayed a semi-mature phenotype, exhibited by low levels of MHC class II and coestimulatory molecules (CD40, CD86) compared to control mature BM-DCs (mBM-DCs) differenciated in the absence of VD3. MOG40-55 loaded TolDCs showed to be poor stimulators of specific T cell proliferation of mice with EAE, compared to mBM-DCs.

Conclusions CONCLUSIONS: These results suggest that MOG-loaded TolDCs may be a powerful tool to induce specific T-cell hyporesponsiveness in EAE. Hence, treatment with tolDCs loaded with myelin peptides might be a potential therapy for EAE/MS. This model can be useful to establish parameters such as dose and route of administration prior to their translation to MS patients.

Authors/Disclosures
PRESENTER	No disclosure on file
	No disclosure on file
	No disclosure on file
Eva Martinez-Caceres	No disclosure on file
Edythe Boitnott	No disclosure on file

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