Abstract Details

Title Platelet Pro-Inflammatory Activation Correlates with Increased Progression of Carotid Atherosclerosis in the After-Stroke Subjects Despite Secondary Stroke Prevention

Topic Cerebrovascular Disease and Interventional Neurology

Presentation(s) P03 - (-)

Poster/Presentation
Number 177

Objective

Background BACKGROUND: Platelets bridge atherothrombosis and arterial inflammation. P selectin (CD62P) and CD40L, both expressed on activated platelets, recruit and activate leukocytes. Platelet activation correlates with carotid intima-media thickness (IMT).

Design/Methods DESIGN/METHODS: The surface expression of CD62P and CD40L, the platelet derived microparticles (PMPs) and monocyte-platelet aggregate (M-plt) formation using five-colour flow cytometry in 83 subjects on the 3 months after stroke, in 69 age-, gender- and vascular risk factors matched disease controls (DC), and in 38 healthy volunteers (HC) were determined. The mean common carotid intima-media thickness (CCAmeanIMT) and maximal bifurcation IMT (BIFmaxIMT) were assessed twice (3 months after the stroke and after a further 21-month period) using colour Doppler ultrasonography. The difference (?) between both measurements was calculated as the marker of atherosclerosis progression. All study participants excluding HC had been chronically receiving 150 mg aspirin per day as secondary/primary vascular disease prevention.

Results RESULTS: In stroke subjects ?CCAmeanIMT was greater than in HC and did not differ from the values observed in DC (0.112±0.109mm vs 0.019±0.009mm, p<0.001; vs 0.114±0.07mm, p=0.28). In after-stroke survivors ?BIFmaxIMT was greater than in both HC and DC (0.304±0.231mm vs 0.056±0.014mm; vs 0.089±0.071mm, p<0.001). Using multiple regression analysis we found that after adjustment according to age, gender, stroke and vascular disease risk factors ?BIFmaxIMT was independently associated with surface expression of CD62P (B=0.17, p=0.04), CD40L (B=0.24, p=0.002), the percentage of M-plt (B=0.18, p=0.02) and PMPs (B=0.25, p=0.006).

Conclusions CONCLUSIONS: The progression of atherosclerosis in carotid bifurcation is increased after the stroke. The platelet activation markers engaged in inflammatory response positively and independently of stroke correlate with progression of carotid bifurcation atherosclerosis despite the prevention with aspirin.

Authors/Disclosures
PRESENTER	No disclosure on file
	No disclosure on file
Wojciech Kozubski, MD, PhD (University of Medical Sciences in Poznan)	No disclosure on file
Mark Hallett, MD, FAAN (National Institutes of Health)	Dr. Hallett has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Janssen. Dr. Hallett has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Neurocrine. Dr. Hallett has received personal compensation in the range of $0-$499 for serving on a Scientific Advisory or Data Safety Monitoring board for Brainsway. Dr. Hallett has received personal compensation in the range of $500-$4,999 for serving on a Scientific Advisory or Data Safety Monitoring board for VoxNeuro. Dr. Hallett has received personal compensation in the range of $5,000-$9,999 for serving on a Scientific Advisory or Data Safety Monitoring board for QuantalX. Dr. Hallett has received personal compensation in the range of $10,000-$49,999 for serving as an Editor, Associate Editor, or Editorial Advisory Board Member for Elsevier. Dr. Hallett has received intellectual property interests from a discovery or technology relating to health care. Dr. Hallett has received publishing royalties from a publication relating to health care. Dr. Hallett has received publishing royalties from a publication relating to health care. Dr. Hallett has received publishing royalties from a publication relating to health care. Dr. Hallett has received publishing royalties from a publication relating to health care. Dr. Hallett has received personal compensation in the range of $500-$4,999 for serving as a Speaker with International Parkinson and Movement Disorder Society. Dr. Hallett has a non-compensated relationship as a Past-President with Functional Neurological Disorder Society that is relevant to AAN interests or activities.

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