Abstract Details

Title Interleukin-1 beta Gene Is Up-Regulated in Both Immature and Adult Zebrafish Brain after Pentylenetetrazole (PTZ) Evoked Seizure

Topic Epilepsy

Presentation(s) P02 - (-)

Poster/Presentation
Number 218

Objective

Background BACKGROUND: Interleukin-1beta (il1b) has shown to be up-regulated in surgical specimens of pharmacoresistent epilepsy patients as well as in experimental rodent models of seizure; however, the profile of il1b in zebrafish brain after seizure is unknown.

Design/Methods DESIGN/METHODS: Zebrafish were maintained according to standard procedures and all experiments were approved by animal ethical committee. Adult zebrafish and larvae at 7 days post fertilization were separated in seizure (SG) and control (CG) groups. Animals from SG group were individually exposed to 15mM PTZ (2-3min for adults and 20min for larvae). Animals from CG were exposed to the same handling condition, but in PTZ-free water. A total of n=5 samples were used for each group. Each adult sample was composed by pooling two brains and 20 heads were used to compose each larvae sample. At the time 0.05h and 6h after seizure insult, animals were anesthetized and their brains were immediately frozen in N2(L) and total RNA extracted by Trizol. Reverse transcriptase quantitative-PCR amplifications were carried out in 7500 Real Time Fast PCR System with TaqMan® gene expression assays. Runs were in triplicates using bact as endogenous control. The relative quantification (RQ) was calculated by the equation RQ=2 -??CT. Statistical analysis was performed by Mann-Whitney test (p? 0.05).

Results RESULTS: PTZ treatment up-regulated mRNA profile of the il1b gene at 0.05h and 6h in adult zebrafish brain but only at 0.05h in immature brain.

Conclusions CONCLUSIONS: Our results show an up-regulation of il1b mRNA levels after PTZ-evoked seizure in immature and adult zebrafish brain. These findings show that zebrafish has a similar pattern as observed in rodents providing new insights about this little fish as a model for epilepsy studies.

Authors/Disclosures
Patricia G. Barbalho, PhD PRESENTER	No disclosure on file
	No disclosure on file
	No disclosure on file
Claudia V. Maurer-Morelli, PhD (University of Campinas)	No disclosure on file
Krai Chatamra, PhD (InTrance Medical Systems)	No disclosure on file

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