Abstract Details

Title HLA Typing Using Next Generation Sequencing in Saudi Multiple Sclerosis Patients

Topic MS and Related Diseases

Presentation(s) P05 - (-)

Poster/Presentation
Number 139

Objective

Background BACKGROUND: Several studies reported on the association of different HLA antigens with Multiple sclerosis. Few studies have looked into the high resoluation of HLA associations with MS and non from our region. In apiolt study we have investigated the HLA associations with a Saudi cohort of MS patients using next generation seuencing.

Design/Methods DESIGN/METHODS: Next generation Roche 454 GS FLX Titanium chemistry with combination of HLA primer pairs and multiplex identification (MID) tags for HLA-A, B, DRB1 and DQB1 sequencing was used to analyze MS cases. Analysis of sequence reads was performed using Conexio Assign software. A total of 31MS cases were studied, their HLA allele frequencies were compared to 383 healthy controls.

Results RESULTS: The Roche GS FLX HLA system yielded an average amplicon read depth of 250. Class I and II genotype calls were assigned and their frequencies were compared to control data. HLA-A*01 (OR 2.96, 95%CI 1.46-6.02, p=0.004), B41 (OR 3.77, 95%CI 1.64-8.63, p=0.0025), and DQB1*03 (OR 2.20, 95%CI 1.29-3.75, p=0.0051) were significantly associated with Saudi MS patients.

Conclusions CONCLUSIONS: This is the first report on HLA association with MS in the Saudi population using high-resolution sequencing. Our results shows high association with the following alleles: HLA-A*01:01, B*41:01 and DQB1*03:02. Our results show unique associations compared to other populations. Surprisingly no HLA-DRB1 associations were found. This work is still in progress as we are analyzing more MS patients' and normal control samples.

Authors/Disclosures
PRESENTER	No disclosure on file
	No disclosure on file
Suleiman Kojan, MD, FAAN (Flint Neurological Center)	No disclosure on file
	No disclosure on file
Ali M. Al Khathaami, MD (National Guard Health Affair)	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
Christine Lebrun Frenay, MD, FAAN (CRCSEP Neurologie)	Dr. Lebrun Frenay has received personal compensation in the range of $0-$499 for serving as an Editor, Associate Editor, or Editorial Advisory Board Member for Revue Neurologique. The institution of Dr. Lebrun Frenay has received research support from FRANCE SEP.
	No disclosure on file

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