Abstract Details

Title Effects of Intraplantar and Intrathecal Botulinum Toxin Type B on Mono- and Poly-Neuropathic Pain in Mouse

Topic Peripheral Nerve

Presentation(s) P02 - (-)

Poster/Presentation
Number 184

Objective

Background BACKGROUND: Intrathecal (IT) botulinum toxin B (BoNTB) yields a potent analgesia in a murine mononeuropathy (MN) model. Recent work has suggested a possible peripheral action of the BoNTs. We extended our initial work examining the effects of IT and intraplantar (IPLT) BoNTB on allodynia in a MN and a poly neuropathy (PN) mice.

Design/Methods DESIGN/METHODS: An MN was prepared by ligating the L5 spinal nerve root. A PN was made by injecting cisplatin (2.3 mg/kg/day, intraperitoneal;IP) every other day for six times. Saline or BoNTB (up to 1 U) were administered once iplt (unilaterally) or once IT, Mechanical allodynia was assessed periodically before and after IPLT/IT delivery. Motor function of the neuropathic mice was assessed by using the hanging test and grip test.

Results RESULTS: MN and PN mice showed a prominent allodynia. IPLT BoNTB resulted in a long lasting (up to 21 day) reversal of the allodynia in the PN and MN models. IT BoNTB resulted in a long lasting (up to 21 day) reversal of the allodynia in the PN model. The antiallodynic effects of BoNTB were blocked by prior reduction with dithiothreitol. BoNTB at efficacious doses did not alter motor function.

Conclusions CONCLUSIONS: IPLT and IT BoNTB yields a persistent attenuation of the allodynia in mouse MN and PN pain models. Wheteer the iplt effects represent a peripheral or central action is unknown.

Authors/Disclosures
PRESENTER	No disclosure on file
Marc Marino	No disclosure on file
	No disclosure on file
	No disclosure on file
Aksel Siva, MD (Istanbul University Cerrahpasa School of Medicine)	Dr. Siva has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Novartis. Dr. Siva has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Roche. Dr. Siva has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Sanofi-Genzyme. Dr. Siva has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Biogen - TR. Dr. Siva has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Alexion. Dr. Siva has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Ali Raif Pharmaceuticals, Turkiye. Dr. Siva has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Sanovel Pharmaceuticals, Turkiye. Dr. Siva has received personal compensation in the range of $500-$4,999 for serving on a Scientific Advisory or Data Safety Monitoring board for Roche. Dr. Siva has received personal compensation in the range of $500-$4,999 for serving on a Scientific Advisory or Data Safety Monitoring board for Abdi Ibrahim Ilac - TR. Dr. Siva has received personal compensation in the range of $500-$4,999 for serving on a Scientific Advisory or Data Safety Monitoring board for Merck Serono . The institution of Dr. Siva has received research support from Turkish MS Society. The institution of Dr. Siva has received research support from The Scientific and Technological Research Council Of Turkey - Health Sciences Research Grants.

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