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Abstract Details

Intraplantar Botulinum Toxin B: Local Effects and Central Transport
Peripheral Nerve
P02 - (-)
181
BACKGROUND: Botulinum toxins may be transported in the primary afferent after subcutaneous delivery. We queried whether intraplantar (iplt) BoNT-B would affect afferent terminal release and spinal activation.
DESIGN/METHODS: In C57Bl6 mice, BoNT-B (1U / 30[micro]L) was injected iplt unilaterally in the paw at intervals before testing. Four issues were addressed. i) Block of local iplt capsaicin evoked plasma extravasation as measured by the concentration of the blue dye in the paw. ii) Effect of iplt BoNTB upon VAMP in the ispi and contralateral DRG by Western Blotting. iii) Effect of iplt BoNTB on the iplt formalin evoked flinching response. iv) Effect of iplt BoNT on spinal Neurokinin 1 receptor internalization (marker of substance P release) and cFOS (+) neurons.
RESULTS: At doses employed, there were no significant changes in hind limb motor function. i) The ipsilateral iplt capsacin evoked extravasation is diminished after iplt BoNTB. ii) Iplt BoNTB reduced ipsilateral, but not contralateral DRG VAMP. iii) Iplt BoNTB prevented the IPLT formalin evoked NK1 internalization (e.g. reflecting a loss of SP release) and a reduction in the increase in cFos (+) neurons in ipsilateral dorsal horn. iv) Iplt BoNT suppressed phase 2 flinching evoked by iplt formalin.
CONCLUSIONS: Iplt BoNT-B pretreatment reduces formalin evoked flinching at doses which blocked local plasma extravasation, reduced spinal sP release and neuron activation. It is not clear whether the analgesia arises from a peripheral or central action, but the effects of IPLT BoNTB on ipsilateral DRG VAMP emphasizes the likelihood of a central transport.
Authors/Disclosures
Marc Marino
PRESENTER 		No disclosure on file
No disclosure on file
No disclosure on file
No disclosure on file
No disclosure on file
Jennifer L. Hopp, MD, FAAN (University of Maryland Medical System) The institution of Dr. Hopp has received research support from MII TA Grant 20180828 TEDCO EpiWatch: A Mobile Health Application for Epilepsy Management. The institution of Dr. Hopp has received research support from MII TA Grant TEDCO Project #0723-012 EPIScalp; A Novel Tool for Diagnosing Epilepsy from Scalp EEG. The institution of Dr. Hopp has received research support from NIH R44NS137845. Dr. Hopp has received publishing royalties from a publication relating to health care. Dr. Hopp has received personal compensation in the range of $5,000-$9,999 for serving as a Faculty with J. Kiffin Penry Epilepsy 好色先生al Programs.