Abstract Details

Title Peripheral Blood Mononuclear Cells - Platelets Aggregates and the Levels of Selectins in Lung Cancer Patients with Consideration of Paraneoplastic Neurological Syndromes

Topic Neuro-oncology

Presentation(s) P02 - (-)

Poster/Presentation
Number 162

Objective

Background BACKGROUND: Leukocytes and platelets co-operate in host-tumor interactions and selectins expressed on both cells types mediate it. PNS result from indirect effects of systemic malignancy and lung cancer is diagnosed as one of the most frequent neoplasms among their causes.

Design/Methods DESIGN/METHODS: We included in the study 68 patients hospitalized in Neurological Clinic with PNS diagnosis and Department of Pulmonology, Allergology and Respiratory Oncology Poznan University of Medical Science with the diagnosis of lung cancer. Definite diagnosis of PNS was based on Graus criteria. Each serum was tested for onconeural antibodies by means of indirect immunofluorescence and confirmed by Western blotting. Serum L-Selectin and P-Selectin were evaluated by means of ELISA.

Results RESULTS: In general 31% of lung cancer patients presented neurological deficit, with classical PNS manifested as subacute sensory neuropathy and paraneoplastic cerebellar degeneration. Among well defined onconeural antibodies we have found anti-Hu, anti-Ri, anti-amphiphysin and anti-Ma/Ta. PBMC-platelets aggregates were observed in 49% of patients. L-Selectin levels were higher in lung cancer patients with PNS symptoms (844+/-319ng/mL) than asymptomatic cases (837+/-198ng/mL; mean+/-SD; P=0.042). When clinical PNS manifestation was taken into consideration L-Selectin was higher (P=0.012) in patients with non-classical PNS (921+/-392ng/mL) comparing to asymptomatic cases (837+/-198ng/mL). In patients with PBMC-platelets aggregates the L-Selectin concentration (952+/-324ng/mL) was increased (P=0.029) comparing to the subjects without aggregates (828+/-201ng/mL). Logistic regression analysis in the model including the effects of L-Selectin, P-Selectin, age and sex on the presence of well-defined onconeural antibodies showed L-Selectin as an independent factor (P=0.0313), and when the influence on neurological deficit was analyzed-P-Selectin was independent factor (0.0481).

Conclusions CONCLUSIONS: L-Selectin up-regulation associates both with PBMC-platelets aggregates formation, with the presence of well-defined onconeural antibodies and non-classical PNS manifestation.

Authors/Disclosures
Slawomir Michalak, PhD PRESENTER	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
Wojciech Kozubski, MD, PhD (University of Medical Sciences in Poznan)	No disclosure on file

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