Abstract Details

Title Fluctuations of Plasma Microbial Translocation Products among Patients at Risk of Chronic HIV-Associated Neurocognitive Disorder

Topic Infections/AIDS/Prion Disease

Presentation(s) P03 - (-)

Poster/Presentation
Number 252

Objective

Background BACKGROUND: Cognitive impairment continues to be an important manifestation in about 15-50% of HIV-infected subjects despite of effective anti-retroviral therapy. Circulating lipopolysaccharides (LPS), derived from the gastrointestinal tract as a result of microbial translocation, is elevated in patients with chronic HIV infection compared to uninfected patients. LPS induces the release of pro-inflammatory cytokines, monocyte activation and enhanced trafficking into the brain all considered to be important mechanism in the pathogenesis of HAND. The degree of MTPs fluctuation within a subject is not known.

Design/Methods DESIGN/METHODS: Two groups of subjects, viremic (undetectable viral load) (n=22) and aviremic (viral load >500 copies /mL) (n=8) were recruited. Blood was collected at 6 time points during the 4-week study. A questionnaire of inter-current illness, anti-biotics use, alcohol intake was administered at each of the 6 visits. Blood samples were analyzed for lipopolysaccharide (LPS), lipopolysaccharide binding protein (LBP), and endotoxin core antibodies levels. Fluctuations of MTPs within individual were calculated as the standard deviation (SD) of all MTPs readings during the study period.

Results RESULTS: The SD of LPS for aviremic subjects ranged between 0 - 12.2 EU/mL, with mean SD of 1.5EU/mL, compared to 0.092- 2.71EU/mL, and mean SD of 0.66 EU/mL in the viremic subjects. The SD of LBP in the aviremic group ranged between 983-49730 ng/mL, with a mean of SD of 7366 ng/mL, while the viremic group ranged between 1993- 12348ng/mL, with a mean of 5455 ng/mL.

Conclusions CONCLUSIONS: There were significant fluctuations of MTPs within individuals, and the magnitude of fluctuation may trend higher in aviremic subjects.

Authors/Disclosures
Ik L. Tan, MD (Sutherland Hospital) PRESENTER	No disclosure on file
Robert P. Lisak, MD, FAAN (Wayne State Univ Sch of Med, Dept of Neuro)	Dr. Lisak has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Argnex. Dr. Lisak has received personal compensation in the range of $500-$4,999 for serving as a Consultant for GLG Consulting. Dr. Lisak has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Third Bridge Consulting. Dr. Lisak has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Clearview Consulting. Dr. Lisak has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Takeda. Dr. Lisak has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Alivar Therapeutics. Dr. Lisak has received personal compensation in the range of $500-$4,999 for serving as a Consultant for ANI Pharmaceuticals. Dr. Lisak has received personal compensation in the range of $0-$499 for serving as a Consultant for Coleman Research. Dr. Lisak has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Horizon. Dr. Lisak has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Immunic. Dr. Lisak has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Guidepoint Consulting. Dr. Lisak has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Tedus Consulting. Dr. Lisak has received personal compensation in the range of $500-$4,999 for serving on a Scientific Advisory or Data Safety Monitoring board for COUR Pharma. The institution of Dr. Lisak has received research support from NINDS. The institution of Dr. Lisak has received research support from Argenx. The institution of Dr. Lisak has received research support from Ra Pharmaceuticals. Dr. Lisak has received publishing royalties from a publication relating to health care. Dr. Lisak has received publishing royalties from a publication relating to health care.
	No disclosure on file
Bryan Smith, MD (DC VA Neurology 3BW)	Dr. Smith has nothing to disclose.
Justin C. McArthur, MBBS, MPH, FAAN (Johns Hopkins University)	No disclosure on file
Carlos A. Pardo-Villamizar, MD (Johns Hopkins U, Med Dept of Neurology)	The institution of Dr. Pardo-Villamizar has received research support from National Institutes of Health. The institution of Dr. Pardo-Villamizar has received research support from Bart McLean Fund for Neuroimmunology Research .

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