Abstract Details

Title Salivary Biomarkers in Huntington’s Disease

Topic Movement Disorders

Presentation(s) P1 - Poster Session 1 (5:30 PM-6:30 PM)

Poster/Presentation
Number 10-041

Objective

The objective of the current study was to assess the potential for saliva to serve as a biospecimen for accessible biomarkers for Huntington’s disease (HD).

Background Peripheral biomarkers are greatly needed in HD to anticipate onset of disease symptoms, monitor progression, and track potential therapeutic effects. Pathogenesis in HD is associated with expression of the mutant Htt (mHtt) protein in the CNS; several therapeutic approaches directed at its production, processing, and/or turnover are under development. Recently, inflammatory molecules have been studied for various neurodegenerative diseases due to their role in neurodegeneration and immune system activation.

Design/Methods We measured total Htt (tHtt) protein in saliva from 98 manifest HD, gene-positive pre-manifest (PM) HD, and age- and sex-matched control (NC) subjects. Additional salivary analytes, including inflammatory markers such as cortisol, C-reactive protein (CRP), interleukin 6 (IL-6) and interleukin 1B (IL-1B), were also measured using standardized ELISAs.

Results Levels of tHtt were significantly higher in saliva from HD patients compared to NC (p=0.02). Salivary tHtt showed no gender effects but showed a significant positive correlation with age, but not age-of-onset or CAG-repeat length. Importantly, salivary tHtt was significantly correlated with several clinical measures-–positively correlated with UHDRS TMS (r=0.374, p=0.038); negatively correlated with UHDRS TFC score (r=0.345; p=0.057); and negatively correlated with SDM score (r=-0.424; p=0.017). CRP, a marker of systemic inflammation, was significantly elevated in saliva in PM subjects compared to NC (p=0.032) and may be an early marker for disease onset. Levels of salivary cortisol (p=0.047) and IL-1B (p=0.021) were significantly higher in HD compared to PM subjects. Levels of salivary IL-6 were significantly higher in HD compared to both PM (p=0.048) and NC (p=0.024) subjects.

Conclusions Measurements of salivary Htt and other salivary proteins offer significant promise as relevant, non-invasive biomarkers of symptom onset and disease progression in HD.

Authors/Disclosures
Jody Corey-Bloom, MD, PhD, FAAN (UCSD Neurosciences) PRESENTER	Dr. Corey-Bloom has received personal compensation in the range of $500-$4,999 for serving on a Scientific Advisory or Data Safety Monitoring board for UniQure. Dr. Corey-Bloom has received personal compensation in the range of $500-$4,999 for serving on a Speakers Bureau for Teva Pharmaceuticaks. Dr. Corey-Bloom has received personal compensation in the range of $10,000-$49,999 for serving as a Co-Director, HD-Net with Huntington Study Group.
	No disclosure on file
Ameera Haque	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file

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