The effects of mechanical impact forces on neurological health are a critical concern, likely due to issues of traumatic brain injury (TBI) in sports and brain damage stemming from the potential of "sonic terrorism." The quantitative analysis and evaluation of such forces on brain tissue function is very difficult. To address this issue, this research proposes a novel approach of using a cellular model subjected to mechanical vibration for analysis. Here, neuron-like differentiated neuroblastoma cells were subjected to vibration at frequencies of 20, 200, 2000, and 20000 Hz for a period of 24 hours at constant amplitude. Cell proliferation, cytokine production, including inflammatory cytokines IL-6, IL-1β, TGF- β1, and hypoxia-induced cytokine VEGF, and neurite outgrowth were assayed as response of the cells and indicators of cellular health after vibrational treatment. Cell proliferation was found to increase after 20, 200, and 20000 Hz treatments; p<0.05) and decrease after 2000 Hz treatment (p<0.05). IL-6 production was found to decrease after 200 and 20000 Hz treatments (p<0.01) and increase after 20 and 2000 Hz treatments (p<0.01). IL-1β protein production was found to decrease after 20 Hz and increase after 200 Hz treatments (p<0.001), while TGF- β1 was found to decrease after 200 Hz treatment (p<0.001). VEGF production was found to increase after 2000 Hz treatment (p<0.05) and decrease after 20000 Hz treatment (p<0.05). After staining, longer neurites and greater neurite networking were observed at proliferative frequencies, and shorter neurites with more discrete cells were observed at 2000 Hz. The results suggest that cell proliferation, cytokine production, and neurite outgrowth serve as a sensitive measure to external impact forces applied to the cells. In addition, it is suggested that inflammatory mechanisms exhibit inhibitory "cross-talk" between IL-6 and IL-1β signaling pathways at 20 and 200 Hz along with "cross-talk" between inflammatory and hypoxia-induced mechanisms. Cytokine data suggest frequency-specific responses, which can be used not only to better understand the mechanism of vibration induced cellular damage, but also to unveil the cellular signaling processes.