Abstract Details

Title Age Related Responses to Injury and Ensheathment Capacity of Human Oligodendrocytes

Topic Multiple Sclerosis

Presentation(s) P3 - Poster Session 3 (12:00 PM-1:00 PM)

Poster/Presentation
Number 9-004

Objective Compare the susceptibility to metabolic stress mediated injury and ensheathment capacity of mature OLs and A2B5 antibody reactive cells (termed pre-OLs or A2B5+ cells) isolated from pediatric and adult surgically resected brain tissue samples.

Background White matter injury can result from multiple insults to the CNS including hypoxia/ischemia and inflammation across different age groups. Restoration of myelination can involve both previously myelinating oligodendrocytes (OLs) and recruitment of OL progenitor cells. Histologic and experimental model studies indicate that donor age and differentiation stage of OLs can influence injury and repair responses.

Design/Methods

OLs were isolated from surgically resected “normal appearing” pediatric (ages 2-14) and adult (ages 40-65) brain tissues using a percoll gradient/overnight cell adhesion technique (Cui, 2017). Pre-OLs were selected from floating cells using immune-magnetic beads coated A2B5 antibody. Cell stress response was assessed after 48 hours by determining cell death (% propidium iodide (PI)+ cells) in dissociated cell cultures under glucose deprivation (NG)conditions. Ensheathment capacity was measured by adding OLs or A2B5+ cells to artificial nanofiber plates for 3-4 weeks (Xu, 2019).

Results

Under NG conditions, the % PI+ cells for OLs was increased by 2.3 fold after 2 days for pediatric samples and 1.5 for adults. % PI+ was higher for A2B5+ cells than total OLs for both groups. % nanofiber ensheathing cells was higher in A2B5+ cells from both age groups compared to OLs. Ensheathment by A2B5+ cells and OLs was greater in pediatric versus adult samples.

Conclusions OLs and their precursors, isolated from pediatric samples are more susceptible to metabolic stress related cell death but show enhanced ensheathment capacity when compared to cells isolated from adults. Defining the basis for these age-related differences can guide the development of neuroprotective and repair directed therapeutic strategies.

Authors/Disclosures
Jack P. Antel, MD, FAAN (Montreal Neurologic Hospital) PRESENTER	Dr. Antel has received personal compensation in the range of $5,000-$9,999 for serving on a Scientific Advisory or Data Safety Monitoring board for sanofi, roche, biogen, wave,nervgen. The institution of Dr. Antel has received research support from Novartis canada bristol myers squib. Dr. Antel has a non-compensated relationship as a past president with ACTRIMS that is relevant to AAN interests or activities.
Qiao Ling Cui, MD	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
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	No disclosure on file
	No disclosure on file

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