Abstract Details

Title Role of ACE Polymorphism in Acute Ischemic Stroke

Topic Cerebrovascular Disease and Interventional Neurology

Presentation(s) S33 - Cerebrovascular Disease: Basic Sciences and Cohort Studies (3:42 PM-3:54 PM)

Poster/Presentation
Number 002

Objective To investigate the role of ACE insertion/deletion (I/D) polymorphism in acute ischemic stroke (AIS) in the North Indian population.

Background

Stroke is one of the leading causes of death and disability in India. Angiotensin-Converting Enzyme (ACE) is involved in the development of hypertension, atherosclerosis, cardiovascular, cerebrovascular disease, and is a plausible genetic candidate for stroke. Due to conflicting evidence, the role of ACE polymorphism is still uncertain in the Indian population and requires further investigation.

Design/Methods One hundred thirty AIS cases and 130 age and sex matched healthy controls were recruited. Ten milliliters (ml) venous blood was drawn and 6 mL of blood was used for routine hematological, biochemical tests. The remaining four mL of EDTA blood was used for DNA extraction, PCR amplification, and restriction digestion. Three genotypes (II, ID, and DD) were visualized on 3% agarose gel. The association between genotypes among stroke cases and controls was done by Chi-square test using dominant and recessive models. Multivariate logistic regression analysis was done in order to independently assess the association between DD genotype and occurrence of AIS. P-value <0.05 was taken as significant.

Results DD genotype was significantly associated with the risk of stroke with P value of 0.0001. Both the dominant and recessive models showed that the DD genotype was independently associated with an increased risk of ischemic stroke (OR = 20.732; 95% CI: 2.7241–157.7864; P value = 0.003 for the recessive model and OR = 2.848; 95% CI: 1.5127–5.3649; P value = 0.001 for dominant model).

Conclusions Our study showed a strong association between ACE polymorphism and the risk of AIS. This study paves the way for further studies to confirm the role of ACE polymorphism as genetic risk factors for AIS.

Authors/Disclosures
Alvee Saluja, DM (LHMC NEW DELHI) PRESENTER	Dr. Saluja has nothing to disclose.
Ankit Goyal	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file

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