Abstract Details

Title Temporal Evaluation of MicroRNA Expression profile in Acute Anterior Circulation Large Vessel Occlusion Stroke

Topic Cerebrovascular Disease and Interventional Neurology

Presentation(s) S33 - Cerebrovascular Disease: Basic Sciences and Cohort Studies (5:06 PM-5:18 PM)

Poster/Presentation
Number 009

Objective To evaluate the pattern of temporal evolution of circulating exosomal microRNAs (miRs) in acute anterior circulation large vessel occlusion(acLVO) stroke.

Background We lack a reliable surrogate biomarker of stroke lesion age. Between unreliable history and wake up strokes, over 3 million acute ischemic stroke (AIS) patients present with unknown onset annually. Recently, several miRs have been reported as AIS biomarkers but studies specific to acLVO stroke are lacking. It is unclear how miRs evolve over time and whether certain miRs or clusters correlate with lesion age.

Design/Methods After IRB approval, acLVO patients aged 18-90 were prospectively enrolled within 6 hours(h) of onset. Blood sample were collected at 4 time points from onset (0-6h; 6-12h ;12-24h; and 5-7 days). Blood from healthy volunteers were collected once. We conducted pilot study on 36 plasma sample (7 patients and 8 volunteers). After exosome and total RNA isolation, Nanostring assay was used for miR profiling. Counts were normalized and fold changes (FC) were calculated (FC >2 considered significant).

Results Using ANOVA, we identified 11 miRs ( miR 7-5p, miR 378i, miR 365b-5p, miR 140-5p, miR320e, miR 122-5p, miR 448, miR-1258, miR 26a-5p, miR 28-59 & miR 510-3p) with significant differential expression (p<0.05) between any 2 time points (6 vs 24h, 6 vs 5-7 day, 12 vs 5-7day, 24 vs 5-7 day). Three clusters of miRs with similar temporal expression pattern were identified using Pearson correlation of each miR against every other miR. On comparing stroke time points with volunteers, there were 21 miRs with p <0.1 but none with p <0.05. Pathway enrichment analysis of mRNA targets of significantly expressed miRs demonstrated known links to stroke

Conclusions Expression profiles of circulating exosomal miRs in acLVO stroke demonstrate clear temporal evolution. Further analysis on a larger sample is needed to investigate the role of miR as surrogate stroke clock

Authors/Disclosures
Shraddha Mainali, MD (Virginia Commonwealth University) PRESENTER	Dr. Mainali has received personal compensation for serving as an employee of Virginia commonwealth University.
	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
Daniel Woo, MD, FAAN (University at Buffalo)	The institution of Dr. Woo has received research support from NIH.

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