Abstract Details

Title Immunoreactivity and A New Staining Method of MCT-1 Located in Endothelial Cells of Cerebral Vessels of Human Brain in Distinguishing Cerebral Venules from Arterioles

Topic Cerebrovascular Disease and Interventional Neurology

Presentation(s) P5 - Poster Session 5 (11:45 AM-12:45 PM)

Poster/Presentation
Number 13-002

Objective We aimed to immunohistochemically assess the monocarboxylate transporter 1, a specific marker of venous endothelium found in rodent studies, in different caliber vessels in human brains.

Background Although in pressing need, neuropathological studies on venules are scarce, likely owing to limited histological methods in identifying cerebral venules. Distinguishing brain venules from arterioles with arteriolosclerosis is less reliable using traditional staining methods. Recently, Michael et al revealed the molecular definitions of vascular arteriovenous types using single-cell transcriptomics in adult mice, and identified that monocarboxylate transporter 1 (MCT1), encoded by solute carrier 16a1 (Slc16a1), is an underlying endothelial venous and capillaries marker, whereas it remains to be clarified in human brains.

Design/Methods Both large- and small-caliber cerebral vessels were dissected from four autopsy donors. Immunoreactivity for monocarboxylate transporter 1 was examined in all autopsied human brain tissues, and then each vessel was identified by neuropathologists using hematoxylin and eosin stain, the Verhoeff’s Van Gieson stain, immunohistochemical stain with antibodies for α-smooth muscle actin and monocarboxylate transporter 1 in sequence.

Results A total of 61 cerebral vessels, including 29 arteries and 32 veins were assessed. Immunoreactivity for monocarboxylate transporter 1 was observed in the endothelial cells of various caliber veins as well as the capillaries, whereas that was immunenegative in the endothelium of arteries. The different labeling patterns for monocarboxylate transporter 1 could aid in distinguishing various caliber veins from arteries, whereas assessment using the vessel shape, the internal elastic lamina, and the pattern of smooth muscle fibers failed to make the distinction between small-caliber veins and sclerotic arterioles.

Conclusions Monocarboxylate transporter 1 immunohistochemical staining is a sensitive and reliable method to distinguish cerebral veins from arteries.

Authors/Disclosures
Yuan Cao (PUMCH) PRESENTER	Ms. Cao has nothing to disclose.
Dong-Hui Ao	No disclosure on file
	No disclosure on file
	No disclosure on file
Yi-Cheng Zhu, MD, PhD (Peking Union Medical College Hospital)	Dr. Zhu has nothing to disclose.

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