15 male mice (C57BL/6J breed) in each age category, 80 and 10 weeks old. Each receive chemical induced trauma to their right quadricep through barium chloride injection. Quadricep tissue is extracted and collected at five timepoints (day zero, one, three, seven, and fourteen), notable within the regenerative and repair process. RNA is extracted, purified from the tissue, then qRT-PCR is conducted, a method that quantifies changes in gene expression, allowing for the differentiation between the two groups. Using ImageJ, histopathological images of uninjured myofibers are taken to obtain the values of cross-sectional areas per myofiber. 

The sample size may have negatively affected the results because it was too small to accurately represent the whole. Even so, myogenic genes are still shown to have been expressed less in aged mice, possibly indicating that these genes may play a more important role in the regenerative process.