Abstract Details

Title Cerebrospinal Fluid Biomarkers in Idiopathic Intracranial Hypertension: An Exploratory SWATH Proteomics analysis.

Topic Neuro-ophthalmology/Neuro-otology

Presentation(s) P17 - Poster Session 17 (11:45 AM-12:45 PM)

Poster/Presentation
Number 2-005

Objective

To identify CSF biomarkers in Idiopathic Intracranial Hypertension (IIH) cases compared to control subjects using an untargeted SWATH-MS proteomics approach.

Background

The pathogenesis of Idiopathic Intracranial Hypertension (IIH) is currently poorly understood. Speculations regarding the role of cerebrospinal fluid (CSF) protein biomarkers in understanding the pathomechanism of IIH need to be studied using the high-throughput proteomics approaches.

Design/Methods This case-control study was conducted in the Department of Neurology, All India Institute of Medical Sciences, New Delhi, India. CSF samples were collected prospectively from Idiopathic Intracranial Hypertension (IIH) cases and control subjects comprising of non-head injury trauma cases and degenerative joint diseases patients undergoing spinal anesthesia which were subjected to SWATH-MS based untargeted proteomics. Proteins upregulated>1.5 fold and downregulated<0.67 fold with a p-value<0.05 were considered significantly differentially expressed between the two groups. Protein interaction network analysis was performed using STRING and functional analysis was performed using g:Profiler. Statistical analysis was conducted in R version 3.6.2.

Results Our study included CSF samples from 33 subjects, consisting of 13 Idiopathic Intracranial Hypertension (IIH) cases and 20 controls with a mean age of 27.69 (6.94) and 37.35 (13.72) respectively. Total 262 protein groups were identified in Proteinpilot^TM search in 1% global FDR. Through SWATH analysis, we could quantify 232 proteins. We observed 37 significantly differentially expressed proteins between the two groups with 24 upregulated and 13 downregulated proteins in IIH cases compared to controls. The network for 23 proteins was highly connected in the interaction analysis.

Conclusions Neurosecretory, neuroendocrine and inflammatory proteins were predominantly involved in causing Idiopathic Intracranial Hypertension (IIH). This pilot exploratory study served as a platform to identify 37 differentially expressed proteins in IIH. These proteins should further be validated in a large set of patients to confirm their role in the pathogenesis of IIH.

Authors/Disclosures
PRESENTER	No disclosure on file
Shubham Misra, PhD (Yale University)	Dr. Misra has nothing to disclose.
	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
Achal K. Srivastava, MD, FAAN (AIIMS)	Dr. Srivastava has nothing to disclose.
Deepti Vibha, MD (All India Institute of Medical Sciences)	Dr. Vibha has nothing to disclose.
Ajay Garg	Ajay Garg has nothing to disclose.
	No disclosure on file

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