Abstract Details

Title Cerebrovascular Extracellular Vesicles are Increased in Acute- and Chronic-Cerebrovascular Events and Associate with Cognitive Impairment

Topic Cerebrovascular Disease and Interventional Neurology

Presentation(s) P3 - Poster Session 3 (11:45 AM-12:45 PM)

Poster/Presentation
Number 13-009

Objective Determine plasma concentration of cerebrovascular endothelial extracellular vesicles (CEEVs) between controls (CN) and patients with acute- and chronic-cerebrovascular events. Secondarily, we evaluated cognitive performance in our sample to assess the relationship of CEEVs and cognition.

Background Endothelial extracellular vesicles (EEVs) are known to amplify during insult and carry cargo that can have deleterious effects on target cells. We previously showed that increased CEEVs significantly associated with impaired cognitive performance and subclinical load of small vessel ischemic lesions. However, it is unknown whether CEEVs are elevated in acute- and chronic-cerebrovascular events.

Design/Methods CEEV concentration and ratio (CEEVs/systemic EEVs) were conducted by nano-flow cytometry and cognition was assessed by Montreal Cognitive Assessment (MoCA). Controls with no history of stroke or cognitive impairment (CN: n=8, 68.9 ±5.8 years old (y/o), 50% females), patients with chronic cerebrovascular events (CCE) of any etiology, hemorrhagic or ischemic (n=4) or history of transient ischemic attack (n=2) >1 month (CCE: 70.5 ±9.3 y/o, 50% females) and acute cerebrovascular events (ACE) of any etiology, hemorrhagic or ischemic (ACE: n=5, 47.4 ±10.0 y/o, 60% females) were included in data analysis. MoCA was conducted in all CN (27.6 ±1.1) and CCE patients (19.5 ±4.9) and two ACE patients (25.1 ±5.0).

Results Kruskal-Wallis’s test with Dunn’s correction showed increased ratio of CEEVs in CCE (median=12%, IQR=[7.5-30.12], p=0.04) and ACE (median=20.38%, IQR=[11.5-35.1], p=0.02) compared to CN (median=3.4%, IQR=[2.5-7.1) with no difference between CCE and ACE. Spearman correlation determined a moderate negative relationship with a trend towards significance between CEEV ratio and cognition (n=16, r=-0.46, p=0.08).

Conclusions

These data support our previous findings of CEEVs as markers of cognitive impairment and expand the association between CEEVs and subclinical small vessel lesions to acute- and chronic-cerebrovascular events. Determining the mechanistic underpinning for continued CEEV potentiation from acute- to chronic-stroke may provide a therapeutic route for mitigating post-stroke cognitive impairment.

Authors/Disclosures
Cameron Owens, MS PRESENTER	Mr. Owens has nothing to disclose.
Camila Bonin Pinto (Oklahoma University)	Camila Bonin Pinto has nothing to disclose.
Eva Troyano Rodriguez, PhD	Dr. Troyano Rodriguez has received personal compensation for serving as an employee of Oklahoma Medical Research Foundation.
Zsofia Szarvas, MD	Dr. Szarvas has nothing to disclose.
Laura Boada-Robayo, MD, Research Fellow (Hospital Universitario Mayor - Méderi)	No disclosure on file
Demian Rudyk, MD	Dr. Rudyk has nothing to disclose.
Stefano Tarantini, PhD	Dr. Tarantini has nothing to disclose.
Calin I. Prodan, MD (Univ of Oklahoma - Neurology Dept)	The institution of Dr. Prodan has received research support from US Department of Veterans Affairs (Merit award CX000340).
Andriy Yabluchanskiy	Andriy Yabluchanskiy has nothing to disclose.
Faddi G. Saleh Velez, MD (University of oklahoma health Sciences center)	Dr. Saleh Velez has nothing to disclose.

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