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Abstract Details

Automated Behavioral Quantification of Scn1b Null Mice Using LabGym: A Platform for High-throughput Phenotyping of Epilepsy
Child Neurology and Developmental Neurology
P3 - Poster Session 3 (5:00 PM-6:00 PM)
8-009

To develop and validate a deep-learning-based behavioral quantification approach using the open-source LabGym AI platform to identify and characterize motor seizures in the Scn1b null mouse model of Dravet syndrome.

Animal behaviors and movements have traditionally been analyzed manually. Recently, an open-source AI platform, LabGym, was developed to provide an integrated platform for the holistic assessment of animal behavior. We applied LabGym to identify seizures in a well-characterized mouse model of developmental and epileptic encephalopathy. By allowing for a flexible recording background and training on pre-recorded video data, this approach increases the throughput of seizure analysis in mouse models of epilepsy.
Neonatal Scn1b null mice were previously recorded exhibiting characteristic motor seizure activity. A LabGym Detector model, which tracks individual animals, was trained to accurately detect mice during the recording. A LabGym Categorizer, which classifies behaviors, was trained to identify standing, running, sniffing, turning, and seizure behaviors.
Preliminary LabGym classifiers achieved a 94% precision in detecting seizure-like behaviors in Scn1b null mice with a 95% accuracy across all 5 behavior classifiers. The model reliably identified overt motor seizures.
This work demonstrates LabGym as a novel method for quantifying complex behaviors, including motor seizures, in Scn1b null mice. Further refinement of the model, including incorporation of subtle seizure types, will enable the development of a robust, scalable tool for mouse epilepsy phenotyping.
Authors/Disclosures
Joshua M. Adams, MD, PhD (University of Michigan)
PRESENTER 		Dr. Adams has nothing to disclose.
Robert J. Tomlinson Mr. Tomlinson has stock in Johnson & Johnson.
Alexander Junxiang Chen Mr. Chen has nothing to disclose.
Lori L. Isom, PhD Dr. Isom has stock in Abbvie. The institution of Dr. Isom has received research support from NIH. The institution of Dr. Isom has received research support from CURE Epilepsy. The institution of Dr. Isom has received research support from Dravet Syndrome Foundation.
Bing Ye, PhD The institution of Prof. Ye has received research support from NIH.